Porcine anterior segment perfusion culture and trabecular meshwork organ culture
- VernacularTitle:猪眼小梁组织眼前节灌流培养和组织块培养
- Author:
Qi ZHOU
;
Yuqin LIU
;
Jialiang ZHAO
;
Hua ZHANG
- Publication Type:Journal Article
- Keywords:
ocular trabecular meshwork;
anterior segment perfusion culture;
TM organ culture
- From:
Basic & Clinical Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To verify the application of anterior segment perfusion culture and trabecular meshwork (TM) organ culture for glaucoma study. Methods TM tissue was cultured by perfused anterior segment and TM organ culture, light microscopy was used to observe the TM cells and intercellular spaces.Results IOP of the porcine anterior segments perfused under constant flow at 0.1 mL/h could bekept in normal range(10～12 mmHg). The IOP was elevated with the increasing of perfusion rate, while the morphology and structure of the tissue were well preserved. TM cultured by TM organ culture could also reserve the tissues well, but the intercelluar spaces collapsed. Conclusion Anterior segment perfusion model could be a short-term high-pressure model and may simulate the normal physical state. Adequate perfusion was necessary for normal TM.
