p21-Activated Kinase 2 Involved in Cytokinesis Independent of Cdc42 During Xenopus Oocyte Maturation
- VernacularTitle:爪蟾p21活化激酶2参与卵母细胞胞质分裂过程不依赖于Cdc42
- Author:
Daye CHENG
;
Bin LIANG
;
Feng LI
- Publication Type:Journal Article
- Keywords:
p21-activated kinase, Xenopus, oocyte, cytokinesis, polar body, Cdc42
- From:
Progress in Biochemistry and Biophysics
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
p21-Activated kinases including p21-activated kinase 2 contributed to the regulation of actin cytoskeleton and cell dynamics. In order to investigate the function of PAK2 on the maturation of Xenopus oocyte, PAK2-NT(PAK2-N-terminal,PAK2-NT) and PAK2-NTm (PAK2-N-terminal mutation) mRNA were microinjected into Xenopus oocyte respectively. Under fluorescent microscopy germinal vesicle breakdown was observed during cytokinesis. To further observe the relationship of oocyte cytokinesis, polar body formation and Cdc42 activity, confocal microscopy with time-lapse was employed . As a result, occurrences of germinal vesicle breakdown in oocytes were similar to those oocytes injected with PAK2-NT mRNA or injected with PAK2-NTm mRNA,but no cytokinesis and polar body formation were observed in oocytes injected with PAK2-NT mRNA or PAK2-NTm mRNA. These results indicated that PAK2 involved in Xenopus oocytes cytokinesis and polar body formation independent of Cdc42 activity.
