Drug-Resistance of Multi-resistant Pseudomonas aeruginosa
- VernacularTitle:多重耐药铜绿假单胞菌的耐药性研究
- Author:
Cuifen SHEN
;
Wenjun JIN
;
Licheng DAI
;
Jianfang HE
;
Xiaoxiang ZHANG
;
Huaying MAO
;
Quan WU
;
Ying CHEN
;
Yunsong YU
- Publication Type:Journal Article
- Keywords:
Multi-resistance;
Pseudomonas aeruginosa;
Drug-resistance
- From:
Chinese Journal of Nosocomiology
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the mechanism of drug-resistance of multi-resistant Pseudomonas aeruginosa,and provide the guideline for treatment and control of P.aeruginosa infection in hospital.METHODS Fifty strains of multi-resistant P.aeruginosa were selected with K-B susceptibility method.The three-dimensional method was taken to differentiate the various beta-lactamases.The relative drug-resistance gene was detected by polymerase chain reaction(PCR).RESULTS Among 50 strains of multi-resistant P.aeruginosa,there were 2 strains(4%)producing ESBLs,20 strains(40%)producing AmpC beta-lactamases,and 11 strains(22%) producing ESBLs and AmpC beta-lactamases at the same time.There were 8 positive genes in the detected drug-resistance gene,the most common sources of gene were CTX(56%),OprD(60%) and aac(6′)-Ⅱ(60%),respectively.CONCLUSIONS The main beta-lactamases are AmpC beta-lactamases and the main genotype is CTX in the multi-resistant P.aeruginosa cultured in our area.The main course of imipenem-resistance was deletion of outer membrane proteins,and the aminoglycoside modifying enzyme gene and disinfectant-resistance gene in multi-resistant P.aeruginosa are acquired.In order to reduce the drug-resistance strains and control the infection of P.aeruginosa,antibiotics should be used reasonably according to drug susceptibility testing clinically.
