Phenotype and Genotype of Plasmid-encoded AmpC and Extended Spectrum Beta-lactamases in Klebsiella pneumoniae
- VernacularTitle:肺炎克雷伯菌质粒编码的AmpC酶和超广谱?-内酰胺酶的表型和基因型分析
- Author:
Dingxia SHEN
;
Yanping LUO
;
Jingrong CAO
;
Guang ZHOU
;
Fang TIAN
- Publication Type:Journal Article
- Keywords:
Klebsiella pneumoniae;
Plasmid;
Extended spectrum beta-lactamases;
AmpC;
Genotype
- From:
Chinese Journal of Nosocomiology
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the phenotype and genotype of plasmid-encoded AmpC and extended spectrum beta-lactamases in Klebsiella pneumoniae.METHODS 3-Aminophenylboronic acid(APB) test and ESBLs confirmatory test were used for phenotypic detection of AmpC and ESBLs.Conjugation was conducted in order to understand the spread of plasmid in bacteria.The size and genotype of ampC and ESBL genes were studied by extraction and purification of plasmid,PCR and sequencing analysis.RESULTS A plasmid of about 15kb was extracted from K.pneumoniae.This plasmid carrying resistance genes to antibiotics could be spread from K.pneumoniae to recipient Escherichia coli NK5449 through conjugation.DHA-type ampC gene and SHV-type ESBLs gene could be amplified from plasmids extracted from both K.pneumoniae and its conjugant in E.coli,they were DHA-1 ampC gene and SHV-12 ESBLs gene confirmed by sequencing analysis.CONCLUSIONS DHA-1 ampC gene and SHV-12 ESBLs gene are detected from the plasmid of K.pneumoniae.
