Molecular Epidemiology of Ureaplasma urealyticum in Patients with Urogenital Infections
- VernacularTitle:泌尿生殖道解脲脲支原体感染的分子流行病学研究
- Author:
Xiangqun YE
;
Yingmu CAI
;
Xiaoyang JIAO
;
Yinge WU
- Publication Type:Journal Article
- Keywords:
Ureaplasma urealyticum;
Biovar;
Genotype
- From:
Chinese Journal of Nosocomiology
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the Ureaplasma urealyticum infection in patients with urogenital infections and the distribution of its serotypes and genotypes.METHODS The clinical samples were firstly screened with B-Merieux mycoplasma ID2 culture and identification kit,then the positive cultural samples were subtyped by PCR method.RESULTS The positive rate of U.urealyticum with cultural kit was 37.43%(670/1790).The 392 positive cultural samples were subtyped with PCR,the positive rate of U.parvum was 71.94% and the positive rate of U.urealyticum was 29.85%.The serotypes of 264 U.parvum positive samples were identified with PCR,there were 20 samples with serovar 1(7.58%),139 samples with serovar 3 or 14(52.65%),and 51 samples with serovar 6(19.32%).Ninety nine positive samples with U.urealyticum were genotyped,the results showed that the rate of genotype 3(serovar 7 or 11) was lower than genotypes 1 and 2.CONCLUSIONS PCR identification and subtyping of Ureaplasma in patients with urogenital infections showed that the major epidemic pathogen of Ureaplasma in east of Guangdong Province is U.urealyticum serovar 3 and serovar 6,and the distribution of different serotypes or genotypes of Ureaplasma in this area is specific.
