Study on immunosensor of immobilized HRP-HBV antibody on Au colloid
- VernacularTitle:纳米金固定酶标抗体的免疫传感器
- Author:
Liu QIN
;
Zhongming LIU
;
Xiaoyong ZOU
- Publication Type:Journal Article
- Keywords:
self-assembled;
Au colloid;
immunosensor
- From:
Chinese Medical Equipment Journal
1993;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To form Hepatitis B electrochemical immunosensor for the clinic detection. Methods Using the gold electrode as the fundus,the thionine and the HBV horseradish peroxidase(HRP) antibody were electrostaticly bounded to the self-assemble monolayer competitively to form the Hepatitis B electrochemical immunosensor. Cyclic voltammetry and linear scan voltammetry were used to investigate the chemical characteristics of the immunosensor. Results The linearity of Hepatitis B in the range of 1:30～1:200 with the correlation coefficient of 0. 994 was obtained. Conclusion The Hepatitis B electrochemical immunosensor formed by this way can be used to the clinic detection.
