Isolation and identification of hepatic stem cells from rat fetal liver in vitro
- VernacularTitle:大鼠胎肝干细胞的体外分离、培养与鉴定
- Author:
Limei QU
;
Xiuying ZHANG
;
Xinrui WANG
;
Fangge DENG
;
Yulin LI
- Publication Type:Journal Article
- Keywords:
rats,Wistar;
hepatic stem cells;
cell culture;
alphafetoproteins;
keratin
- From:
Journal of Jilin University(Medicine Edition)
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate and cultivate hepatic stem cells(HSCs) from rat fetal liver in vitro and identify their biological features.Methods Collagenase perfusion method and mechanical cutting method were used to isolate HSCs from rat fetal liver which were then cultivated by H-DMEM containing 10% fetal bovine serum.The cell surface antigen expression of HSCs was observed with immunocytochemical method under confocal laser scanning microscope.Results The isolated HSCs from rat fetal liver grew to monolayer 5 d after cultivation in vitro.They presented pykno-round cells and distinct borderline under the light microscope.After 8 d the cells grew like epithelium.These cells expressed AFP antigen,CK 18 and CK19.Conclusion The cultivated cells are proved to be HSCs and can proliferate quickly in vitro.
