Relationship between activity and virulence of secretory acid proteinase and extracellular phosphlipase of Candida albicans
- VernacularTitle:白色念珠菌分泌型酸性蛋白酶和细胞外磷脂酶活性与其毒力的关系
- Author:
Yan CAO
;
Ju ZHANG
;
Chunrui SHA
;
Yongchen ZHENG
;
Yonghong LIU
- Publication Type:Journal Article
- Keywords:
Candida albicans;
secretory acid proteinase;
extracellular phospholipase;
virulence
- From:
Journal of Jilin University(Medicine Edition)
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between activity and virulence of secretory acid proteinase and extracellular phosphlipase of different Candida albicans isolated from various specimens.Methods 190 strains of Candida albicans were isolated from various specimens(sputum,blood,thrush,wound,secretion of vaginitis) of patients with Candida albicans infection in hospital.Milk-plate medium and egg-yolk medium were used respectively to test the activities of secretory acid proteinase and extracellular phosphlipase of Candida albicans.The suspension of two forms of Candida albicans(5?106CFU?mL-1) were injected to vein of tail in mice respectively in virulence test.The mortality and mean survival time of mice were observed in 1 month.The virulence was appreciated with the mean survival time of mice.Results All of 190 strains,the positive detectable rates of secretory acid proteinase and extracellular phosphlipase were 83.68% and 85.26%,respectively.Animal experimental results showed the activities of secretory acid proteinase and extracellular phospolipase in hyphal forms were significantly higher than that in spore form(P
