Toxic effect of exotoxin A of Pseudomonas aeruginosa on keratocytes
- VernacularTitle:绿脓杆菌外毒素A对兔角膜基质细胞的毒性作用
- Author:
Jilong HAO
;
Fei WANG
;
Hongyan ZHOU
;
Wenxia ZHAO
;
Shuyan GU
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
keratocytes;
exotoxins;
lactate dehydrogenase
- From:
Journal of Jilin University(Medicine Edition)
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the toxic effect of exotoxin A of Pseudomonas aeruginosa on keratocytes.Methods Three-dimensional gels of type I collagen containing rabbit keratocytes were incubated in the presence of different concentrations of exotoxin A(0.1,1.0,10 mg?L-1),cultivated for 24 h at 37℃,the change of keratocytes in morphology was observed under the light microscope,and the amount of lactate dehydrogenase(LDH) was measured by enzyme linked immunosorbent assay(ELISA).Results The LDH contents in different concentrations(0.1,1.0,10 mg?L-1)of exotoxin A groups were higher than that in the group without exotoxin A(P
