Immunoreactivity changes of dipeptidyl peptidaseⅡ in lens in SCR rats with congenital cataract
- VernacularTitle:先天性白内障大鼠晶状体中二肽基肽酶Ⅱ免疫活性分析
- Author:
Hui ZHANG
;
Yi WANG
;
Fengjuan YANG
;
Xiuhong QIN
;
Meisheng ZHAO
- Publication Type:Journal Article
- Keywords:
cataract;
lens;
dipeptidyl peptidaseⅡ
- From:
Journal of Jilin University(Medicine Edition)
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between dipeptidyl peptidaseⅡ(DPPⅡ) and the pathogenesis of congenital cataract.Methods Eighty SCR rats with congenital cataract were randomly divided into four groups:8,10,12 and 14 weeks groups(n=20).Normal Wistar rats at each age were used as control groups.Immunohistochemical experiments using the streptavidin-biotin immunoper-oxidase method were used to observe the immunoreactivity changes of DPPⅡ in lens in SCR rats at different time(8,10,12,14 weeks).Results The immunoreactivities of DPPⅡ in the lens epithelial cells and fibres of cataractous lenses of all ages were higher than those in control groups.At the 12th and 14th week,immunoreactive staining of DPPⅡwas found to extend into the perinuclear region.Conclusion The immunoreactivity of DPPⅡ is enhanced in cataractous rat lenses.DPPⅡ may be participated in the proteolytic modification of lens proteins during cataractogenesis.
