Effect of basic fibroblast growth factor on sheep bone marrow mesenchymal stem cells proliferation and differentiation in vitro
- VernacularTitle:碱性成纤维细胞生长因子对羊骨髓间充质干细胞体外增殖和分化的影响
- Author:
Lei YANG
;
Wei LI
;
Zhenyu JIANG
;
Guanjun WANG
- Publication Type:Journal Article
- Keywords:
bone marrow;
mesenchymal stem cell;
fibroblast growth factor
- From:
Journal of Jilin University(Medicine Edition)
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of basic fibroblast growth factor(bFGF) on sheep bone marrow mesenchymal stem cells(BMSCs) proliferation and differentiation in vitro.Methods The sheep BMSCs were isolated and expanded in vitro and divided into experimental group and control group.bFGF(2 ?g?L-1) was added into cell culture medium of experimental group.In control group,no bFGF was added.The morphological differences of BMSCs between two group were observed.Growth curves and alkaline phosphatase(ALP) activity were assessed.In order to observe the influence of bFGF on differentiation potential of BMSCs,osteogenic and adipogenic inductions were conducted on BMSCs in two groups.Results Fibroblast colonies appeared 1 week after seeding of the mononuclear cells.2 weeks later,more colonies formed and each colony became larger.The cell number was 2.5 times of the control when the first passage cells grew to confluence and the ALP activity in experimental group was lower than that in control group(P
