Gene expressions of IL-1 and TNF-? in bronchoalveolar lavage fluid and lung tissues in mice with pulmonary fibrosis and their significances
- VernacularTitle:实验性肺纤维化小鼠IL-1和TNF-?的动态表达及其意义
- Author:
Junling YANG
;
Jinzhi YIN
;
Qingshan LI
;
Zhongsen MA
- Publication Type:Journal Article
- Keywords:
pulmonary fibrosis;
bronchoalveolar lavage fluide;
interleukin-1;
tumor necrosis factor
- From:
Journal of Jilin University(Medicine Edition)
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the gene expressions of IL-1 and TNF-? in bronchoalveolar lavage fluid(BALF) and lung tissues in mice with pulmonary fibrosis(PF) at various periods and to study the effects of cytokines on pulmonary fibrosis.Methods Forty male mice were randomly divided into two groups:experimental group(n=30) and control group(n=10),and poured respectively by bleomycin solutions and saline and killed on the 3rd,7th,14th,28th and 56th day.RT-PCR method was used to analyse the mRNA expressions of IL-1 and TNF-?.Results ① The expressions of IL-1 mRNA and TNF-? mRNA in BALF in experimental group were higher than those in control group(P
