Influence of toll-like receptor 4 mutation on mice with hemorrhagic shock-induced acute lung injury
- VernacularTitle:Toll样受体4突变对失血性休克致小鼠急性肺损伤的影响
- Author:
Yuying SHEN
- Publication Type:Journal Article
- Keywords:
Acute lung injury;
Hemorrhagic shock;
Toll-like receptor 4;
Gene
- From:
Journal of Medical Postgraduates
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role and its mechanism of Toll-like receptor4(TLR4) mutation on acute lung injury induced by hemorrhagic shock without resuscitation.Methods:The hemorrhagic shock model of 20 toll-like receptor 4(TLR4) mutant mice and 20 wild type mice(CBA) was reproduced by heart puncture.The lung tissues were collected at 0,1,2,4,and 6 hours after hemorrhagic shock for pathologic analysis,TLR4 mRNA was measured by semi-quantitative reverse transcription-polymerase chain reaction and NF-?B activation was determined by electrophoretic mobility shift assay.Results:The lung neutrophil infiltration and erythrocyte effusion in toll-like receptor 4 mutant mice were milder than those in wild type mice.The expression of TLR4 mRNA and activation of NF-?B were increased following hemorrhagic shock in wild type mice,while there was no significant change in TLR4 mutant mice.Conclusion:TLR4 plays an important role in ALI induced by hemorrhagic shock,and TLR4 mutant alleviates ALI.
