Effects of methylprednisolone on pneumocyte apoptosis during pulmonary ischemia/reperfusion injury in rats

VernacularTitle:甲基强的松龙对大鼠肺缺血-再灌注损伤中细胞凋亡的影响
Author: Hairong WU ; Changlai WANG ; Zheng ZHANG ; Haidong QIN ; Yue HUANG ; Shukui WANG ; Yan LI ; Hui CHENG
Publication Type:Journal Article
Keywords: Lung; Ischemia reperfusion injury; Methylprednisolone; Inhibitor kappa B-?; (Apoptosis)
From: Journal of Medical Postgraduates 2003;0(05):-
CountryChina
Language:Chinese
Abstract: Objective:To investigate the effects of methylprednisolone(MP)on pneumocyte apoptosis during lung ischemia/reperfusion injury in rats and to study the possible role of MP in pneumocyte apoptosis.Methods:Forty-two male Sprague-Dawley rats used for unilateral lung ischemia/reperfusion model were randomly divided into three groups:sham operation group(Sh group),ischemia/reperfusion group(I/R group),and methylprednisolone group(MP group).Each group has two subgroups of three hours and six hours.Apoptosis rate in lung tissue was detected by the way of Annexin-V-PI in flow cytometer.Expression of I?B-? in lung was observed by immunohistochemical stain.The index of quantitative assessment of histological lung injury(IQA),the wet to dry weight ratio(W/D),the pathological and ultrastructure changes of lung tissue were measured.Results:Apoptosis rate,W/D,IQA of lung tissue were significantly higher in I/R group than which in Sh group(P