An experimental study on effects of arsenic trioxide on port-site metastasis under carbon dioxide pneumoperitoneum in a murine model
- VernacularTitle:三氧化二砷对CO_2气腹下小鼠肿瘤腹壁戳口种植影响的实验研究
- Author:
Huaduo ZHANG
;
Shibo SUN
;
Xinlei LI
- Publication Type:Journal Article
- Keywords:
CO_2 pneumoperitoneum;
Laparoscope;
Adherence factor (CD_44);
Vascular endothelial growth factor;
Arsenic trioxide
- From:
Chinese Journal of Minimally Invasive Surgery
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of intraperitoneal instillation of arsenic trioxide (As_2O_3) on metastasis of hepatoma cell line H_22 under CO_2 pneumoperitoneum in mice. Methods The experiment included 40 Kunming mice (cleaning inbred strain). A 1 mm trocar was punctured through the middle abdomen, by which 1?106 tumor cells were administered. Pneumoperitoneum was established with carbon dioxide at the pressure of 8 mm Hg over 30 min. Afterwards, the mice were randomly divided into four groups with 10 mice in each group, receiving intraperitoneal instillation with one of the following: normal saline (1 ml), As_2O_3 (2 mg/kg, 1 ml), As_2O_3 (4 mg/kg, 1 ml), or As_2O_3 (4 mg/kg) with heparin (10 U/ml) (1 ml totally). Levels of CD_44 and vascular endothelial growth factor (VEGF) were measured on the 3rd and 7th day after pneumoperitoneum, respectively. And the survival condition, abdominal circumference, body weight, and diameter of metastatic tumor were compared among the four groups.Results Compared with control group, significantly lower expressions of CD_44 and VEGF were observed the other three groups on the 3rd and 7th day after pneumoperitoneum (P
