Increased phosphorylation of P38 MAPK in cerebral tissues of hypoxic preconditioned mice
- VernacularTitle:低氧预适应小鼠脑组织内P38 MAPK磷酸化水平增高
- Author:
Xiangning BU
;
Song HAN
;
Junfa LI
- Publication Type:Journal Article
- Keywords:
Cerebral hypoxic preconditioning;
P38 MAPK;
phosphorylation;
protein expression;
brain.
- From:
Basic & Clinical Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of P38 mitogen-activated protein kinase (P38 MAPK) in the development of cerebral hypoxic preconditioning. Methods Healthy male BALB/C mice weighted as18～20 g were randomly divided into 7 groups as follows: normoxic control (H0), early (H1～H4) and delayed (H5 and H6) hypoxic preconditioned mice groups. SDS-PAGE, Western blot and Gel Doc imagine systems were applied to quantitatively analyze the level of P38 MAPK phosphorylation and protein expression in the brain of mice. Results The phosphorylation levels of P38 MAPK increased in cortex, hippocampus and hypothalamus of mice in both early (H1～H4) and delayed (H5 and H6) hypoxic preconditioned groups, and the statistic significance (P
