Molecule biology identification for bacteria of bacteroides
- VernacularTitle:拟杆菌属细菌的分子生物学鉴定
- Author:
Guoqiang WANG
;
Yu LUO
- Publication Type:Journal Article
- Keywords:
bacteroides;
16S rRNA;
PCR
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identity bacteria of bacteroides group by PCR.Method The universal primers were designed according to the sequence of 16S rRNA,then 13 strains bacteroides and the other non-bacteroides were amplified by PCR. PCR products were then sequenced and analyzed by Blast.Results Thirteen strains of bacteroides were amplified, and the non-bacteroides were not amplified. The pairs of primers had highly specificity.Comparing the gene sequences of the 13 strains,bacteroides with corresponding standard 16S rRNA sequences,the homology was from 81% to 100%.Conclusion The established PCR assay could specially amplify bacteroides and had good sensitivity with 100 genomes/ml.
