Expression of Th1/Th2 cytokines stimulated by CpG-ODN 2216 in lymphocytes of peripheral blood
- VernacularTitle:CpG-ODN2216刺激健康人外周血淋巴细胞T_H1/T_H2细胞因子的表达
- Author:
Junhao CHEN
;
Chao WU
;
Jian OUYANG
;
Yong LIU
;
Guangyu GU
- Publication Type:Journal Article
- Keywords:
CpG-ODN;
Th1/Th2;
cytokine
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the Th1/Th2 polarization of T lymphocytes in human peripheral blood stimulated by CpG-ODN2216,and the secretion of cytokines in supernatant of cultured PBMCs after stimulation of CpG-ODN2216.Methods Human PBMCs were isolated from blood of donors.The PBMCs were incubated with CpG-ODN2216 for 24 hours.Th1/Th2 subsets in the cultured PBMCs were examined by flow cytometry,and IFN-?,IL-2,IL-4 and IL-10 in the supernatant were assayed by ELISA.Results The percentage of Th1 and Tc1 increased significantly after stimulation of CpG-ODN2216 compared with control group (P0.05).Conclusion The Th1 cells and Tc1 cells in T lymphocytes of peripheral blood could be polarizated by CpG-ODN 2216.IFN-? secretion in PBMCs could be induced by CpG-ODN2216.
