Differentially displayed Foxp3 mRNA in peripheral blood from patients with various stages of gastric cancer
- VernacularTitle:不同分期胃癌患者外周血淋巴细胞Foxp3 mRNA表达水平的研究
- Author:
Jian LU
;
Xiangliang YUAN
;
Lisong SHEN
- Publication Type:Journal Article
- Keywords:
Foxp3;
regulatory T cell;
quantitative real-time PCR;
TNM staging
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss Foxp3 expression in peripheral blood from the patients with various stages of gastric cancer by SYBR Green I real-time PCR assay.Methods Foxp3 standard was prepared by conventional RT-PCR and employed to generate quantitative PCR standard curve.The precision,sensitivity and specificity of the assay were measured.SYBR Green I real-time PCR assay was adopted to detect and quantify Foxp3 expression in peripheral blood from 40 gastric cancer patients and 8 normal controls.Results The coding sequence (CDS) of full length Foxp3 was obtained and used as standards.The dynamic range of standard curve of real-time PCR assay was 102-108 copies.The mean coefficient of variation (CV) of inter-and intra-assay was less than 5%.The minimum detectable limit was 280 copies of Foxp3 mRNA.Foxp3 expression level in gastric cancer was significantly higher than that in normal control (P
