Effects of tetrandrine on proliferation and apoptosis of nasopharyngeal carcinoma cell line CNE
- VernacularTitle:粉防己碱对人鼻咽癌细胞株CNE增殖抑制和凋亡作用的研究
- Author:
Yuxia DENG
;
Xinchen SUN
- Publication Type:Journal Article
- Keywords:
Tetrandrine;
Human nasopharyngeal carcinoma cell line;
Apoptosis;
Gene regulation
- From:
Journal of Medical Postgraduates
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of tetrandrine(Tet) on proliferaton and apoptosis of nasopharyngeal carcinoma cell line CNE.Methods:CNE cells were divided into groups and treated by Tet at different concentrations.Inhibitory effects of tetrandrine were determined by MTT assay.The morphologic changes of CNE cells were observed by transmission electron microscope.DNA fragmentations were determined by gel electrophoresis assay.Cell apoptosis was investigated by flow cytometer.The expressions of apoptosis-related genes were detected by retrotranscriptase polymerase chain reaction(RT-PCR).Results:Tetrandrine possessed inhibitory effect on CNE cell proliferation in a concentration-dependent manner(P
