Study of positive and negative magnetic activated cell sorting separation of platelet purification
- VernacularTitle:正负向磁性细胞分选技术纯化血小板方法的比较
- Author:
Yinze ZHANG
;
Wen XIONG
;
Hongyan ZOU
- Publication Type:Journal Article
- Keywords:
Magnetic activated cell sorting(MACS);
Platelet;
Purification
- From:
Chinese Journal of Blood Transfusion
1988;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To gain purified platelets for further research of the immune function of platelet.Methods Platelets were purified by positive(MS) and negative(LD) magnetic cell sorting(MACS) separation.The percentage of activated platelets was detected by flow cytometry and nucleated cell clearance was evaluated by white cell count.Results The percentage of activated platelets before separation was(2.39?1.10)%,and increased to(2.56?1.08)% and(16.76?4.04)%,respectively,after MS and LD MACS.The clearances of nucleated cells after MACS MS and LD were(98.44?0.24)% and(98.47?0.18)%,respectively.The recovery rate of purified platelet after MS and LD was(76.50?1.49)% and(79.20?2.61)%.Conclusion MACS LD method was more suitable for the platelet purification.
