Effect of ~(137)Cs-irradiation on the components of buffy coat
- VernacularTitle:~(137)Cs?射线对白膜不同成分的影响
- Author:
Yan LIU
;
Huahua FAN
;
Yuchun GONG
- Publication Type:Journal Article
- Keywords:
137Cs-irradiation;
Buffy coat;
Neutrophils;
Monocytes
- From:
Chinese Journal of Blood Transfusion
1988;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of buffy coat components after 25Gy 137Cs-irradiation.Methods Lymphocyte proliferation was detected by MTT,the fluorescent intensity of neutrophils was measured as phagocytotic rate by flow cytometry,O2-release was determined by cytochrome C reduction,and cytokine production pre-and post-irradiation was detected by ELISA.Results 25Gy is adequate to inactivate T cells.The percentages of phagocytosis of E.coli before and after irradiation were of no significant difference.The release of O2-increased significantly and monocytes maintained the ability to produce cytokines after irradiation.Conclusion Radiation does affect the components of buffy coat,and neutrophils isolated should be transfused as early as possible.
