Culture and characterization of human fetal hepatocytes
- VernacularTitle:人胚胎肝细胞的体外培养及表型观察
- Author:
Fan YANG
;
Chunjing WANG
;
Xiaoling SU
;
Bei GU
;
Hong ZHANG
;
Yuqin LIU
- Publication Type:Journal Article
- Keywords:
cell culture;
hepatocytes;
fetal;
sodium butyrate
- From:
Basic & Clinical Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Determining the characteristics of human fetal hepatocytes in vitro. Methods Isolating and culturing human fetal hepatocytes by stepwise trypsinization of liver fragment in vitro; collecting the culture medium to determine the secretion of AFP, ALB and the functional enzymes (including ALT, AST, GGT, ALP and LDH) of different generations in culture; determining the expression of cytochrome C by immunohistochemistry; testing the effects of sodium byturate on human fetal hepatocytes. Results Human fetal hepatocytes were polygonal epithelial cells in DMEM medium. They could be maintained for 5.5 months (about 30 passages) in vitro. They secreted ALB and functional enzymes all over their cultivation. Conclusion Human fetal hepatocytes can be maintained keeping function in vitro for several months.
