Different effects of airway epithelial cells transfected by elafin on bacterial biofilm
- VernacularTitle:内生多肽Elafin转染气道上皮细胞对不同细菌生物膜作用的差异
- Author:
Xiaohong NIE
;
Xiangdong ZHOU
;
Changwu HUANG
- Publication Type:Journal Article
- Keywords:
elafin;
biofilm;
P.aeruginosa;
E.coli;
S.epidermidis
- From:
Basic & Clinical Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the different effect of elafin incubated by different bacteria on P.aeruginosa(Pa) bioflim. Methods To cultivate the A549 cells in vitro, the pEGFP-N1-elafin eukaryotic expression vectors have been transfected to the cells by LipofectamineTM 2000. Elafin transfected cells incubated by the supernatant of S. epidermidis (S.epidermidis group), Pa (Pa group) and E.coli (E.coli group) respectively for 24 hours,the A549 cells were transfected. Then the levels of elafin were detected by ELISA and Western blot. To establish the Pa biofilm model in vitro,a rapid silver nitrate staining procedure and scanning electronic microscope (SEM)demonstrated bacterial biofilm. After biofilm carriers were put into each group and incubated for 8 hours, we measured the proportion of bacteria biofilm by silver nitrate staining and observed the structure of biofilm by SEM. Results The Pa and E.coli groups(especially Pa) raised the content of elafin in cells and the level of secretion increasing as compared to the normal group, while the S. epidermidis group had no change. Both silver nitrate staining procedure and SEM demonstrated the prestnce of bacterial biofilms. The the proportion of bacteria biofilm and the structure of BF in Pa and E.coli groups were changed, especially in Pa group. Conclusion There was a specificity for bacteria to induce the express of elafin. The inducing effect of Pa was more significant than that of E.coli.
