Effects of fluvastatin on intercellular adhesion molecule-1 expression induced by C-reactive-protein in human umbilical vein endothelial cells
- VernacularTitle:氟伐他汀对C反应蛋白诱导的人脐静脉内皮细胞细胞间黏附分子-1表达的影响
- Author:
Lin LV
;
Kefeng LU
;
Xinglei ZHU
;
Aihong WANG
;
Ling GUO
- Publication Type:Journal Article
- Keywords:
atherosclerosis;
HUVECs;
CRP (C-reactive protein);
adhesion molecule;
statins
- From:
Basic & Clinical Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of Fluvastatin on the expression of intercellular adhesion molecule-1 (ICAM-1) in human umbilical vein endothelial cells (HUVECs) induced by C-reactive protein (CRP). MethodsThe HUVECs were primary cultured. HUVECs from third to sixth generations were stimulated with different concentrations CRP and at different times. Then the cells were treated with Fluvastatin in different concentration of 10-7,10-6 and 10-5mol/L. The content of ICAM-1 protein was detected with ELISA, the mRNA expression of ICAM-1 was evaluated by RT-PCR. Results In the control group, HUVECs produce ICAM-1 protein and mRNA in low concentrations. In CRP group, the content of ICAM-1 protein was increased significantly (P
