Effects of transforming growth factor 1 and hypoxia on the expression of vascular endothelial growth factor for Hepatoma cells
- VernacularTitle:TGF-?1和低氧对肝癌HepG2细胞表达VEGF的影响
- Author:
Chengjing CHU
;
Xuewen WANG
;
Fang HE
;
Wanjiang ZHANG
;
Fengmei DENG
;
Le ZHANG
- Publication Type:Journal Article
- Keywords:
transforming growth factor ?1;
vascular endothelial growth factor;
hypoxia;
hepatoma cells
- From:
Basic & Clinical Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Vascular Endothelial Growth Factor(VEGF) to the expression in hepatoma cells affected by TGF-?1 and hopxia. Methods HepG2 cells were cultured in vitro, and treated with different doses of TGF-?1 and Cobalt chloride hexahydrate(CoCl2), or with TGF-?1 and CoCl2. The change of VEGF protein and mRNA was detected by immunohistochemistry and semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Results Immunohistochemistry and RT-PCR showed that the expression of VEGF protein and mRNA were significantly higher in TGF-?1 groups or CoCl2 groups than that of control group(P
