Human dermal fibroblasts as a feeder layer promote the growth of human keratinocytes
- VernacularTitle:人成纤维细胞饲养层促进人角朊细胞生长
- Author:
Pengcheng CHE
;
Hong SUN
;
Liucun CHEN
;
Guang YANG
;
Jianzhong CUI
- Publication Type:Journal Article
- Keywords:
fibroblast;
feeder layer;
type Ⅰcollagen;
type Ⅲcollagen;
keratinocyte culture
- From:
Basic & Clinical Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanism of dermal fibroblasts as a feeder layer to support the growth of human keratinocytes. Methods Human dermis fibroblasts were isolated and cultured and then treated with mitomycin-C. The expression of type Ⅰand type Ⅲ precollagen mRNA and relevant protein in feeder layer were examined by RT-PCR and Immunohistochemistry. KCs were cultured both on FB and NIH3T3 feed layer as control, the adhering numbers and the time of fusion were recorded. Results RT-PCR showed an increase of type Ⅰprecollagen mRNA in FB feeder layer as compared with that of normal fibroblasts (P
