Detection and Plasmid Profile Analysis of ESBLs-producing Klebsiella pneumoniae in Burn Patients
- VernacularTitle:烧伤患者感染产超广谱?-内酰胺酶肺炎克雷伯菌检测及质粒图谱分析
- Author:
Ping LI
;
Zhenfang DONG
;
Xingtang YU
;
Xueli BAI
;
Xiaofei SONG
- Publication Type:Journal Article
- Keywords:
Burn patients;
Klebsiella pneumoniae;
Antibiotic resistance spectrum;
ESBLs detection;
Plasmid DNA profile
- From:
Chinese Journal of Nosocomiology
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the biological and molecular biological characteristics of Klebsiella pneumoniae in burn patients in order to give the first hand information for preventing and controlling of hospital acquired infections.METHODS The identification was done by Bio-Merieux ATB expression.Antimicrobial susceptibility test was performed with K-B method.The plasmid DNA was extracted by Alkaline Lysis,and separated by electrophoresis on the gel.The ESBLs detection was based on NCCLS.RESULTS The K.pneumoniae from the burn patients and the environment were sensitive to CIP,FOX and IPM,but showed resistance to the rest 12 antibiotics.The plasmid DNA profile analysis showed 3 types,and the relative molecular mass was approximately 4.7?106,3.6?106 and 2.0?106.The molecular biological characteristics showed these pathogens were ESBLs-producing K.pneumoniae,which was different from the control bacteria.At the same time,the pathogens caused the original infection were detected,and they were accordingly Staphylococcus aureus,Streptococcus pyogenes,and Pseudomonas aeruginosa.CONCLUSIONS The outbreak in burn patients is caused by ESBLs-producing K.pneumoniae,which has the same antibiotic resistance spectrum and plasmid DNA profile.This ESBLs-producing K.pneumoniae has the same origin.The pathogen might be transmitted by the case history clips and the door knobs.It was suggested that something must be done to enhance the antisepsis administration in order to prevent the hospital acquired infection.
