Expression of histone deacetyase 4 in human liver carcinoma cell line Bel-7402 and its significance
- VernacularTitle:组蛋白去乙酰化酶4在人肝癌细胞Bel-7402中的表达及其意义
- Author:
Mei MENG
;
Chunting WANG
;
Junmei JIANG
;
Jicheng ZHANG
;
Jinjiao JIANG
;
Changjun JIN
- Publication Type:Journal Article
- Keywords:
histone deacetyase 4;
histone;
live carcinoma cell;
cell cycle
- From:
Chinese Journal of Cancer Biotherapy
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression of histone deacetyase4 (HDAC4) in human liver carcinoma cell line Bel-7402 and to explore the regulatory effects of HDAC4 on the proliferation and differentiation of Bel-7402. Methods: Carcinoma cells Bel-7402 was treated with different concentrations of sodium phenylbutyrate (SPB), an inhibitor of HDAC4. Expression of HDAC4 mRNA in Bel-7402 cells was analyzed by RT-PCR before and after SPB treatment. Reverse microscope was used to observe the morphological changes of Bel-7402 cells. MTT assay and flow cytometry were adopted to describe the proliferation and cell cycle of Bel-7402 cells. Expression of P27 protein was determined by immunohistochemical method. The statistical analysis was performed using one-way ANOVA and student t test. Results: SPB significantly decreased the expression of HDAC4 in Bel-7402(0.88?0.13) vs (0.12?0.04), P
