Electrical stimulation of paraventricular nucleus relieved gastric mucosal cellular apoptosis induced by gastric ischemia-reperfusion in rats
- VernacularTitle:电刺激大鼠室旁核减轻缺血-再灌注胃黏膜细胞凋亡
- Author:
Li LI
;
Yongmei ZHANG
;
Jianfu ZHANG
;
Weili QIAO
- Publication Type:Journal Article
- Keywords:
paraventricular nucleus(PVN);
gastric ischemia-reperfusion(GI-R);
cellular apoptosis;
BCL-2;
BAX
- From:
Basic & Clinical Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of electrical stimulation of paraventricular nucleus(PVN) on gastric mucosal cellular apoptosis,proliferation,and expression of BCL-2,BAX induced by gastric ischemia-reperfusion(GI-R) and the potential mechanisms of protection of PVN on GI-R injury.Methods After electrical stimulation of PVN,the experimental model of GI-R were established by clamping the celiac artery for 30 min and then reperfusing the artery for 30 min,1 h,3 h,or 6 h respectively.We used immunohistochemistry to detect the gastric mucosal cells apoptosis,proliferation and the expression of BCL-2,BAX.Results Compared with GI-R group,the electrical stimulation of PVN markedly decreased gastric mucosal cellular apoptosis,increased the proliferation,and promoted the protein expression of BCL-2,but markedly inhibited the protein expression of BAX at 30 min,1 h,3 h after reperfusion respectively.Conclusion The protective effect of PVN on GI-R injury is associated with up-regulation of expression of BCL-2 and down-regulation expression of BAX,and so inhibited gastric mucosal cellular apoptosis and promoted proliferation.
