Human umbilical cord blood mesenchymal stem cells differentiated into neuron-like cells in vitro
- VernacularTitle:人脐血间充质干细胞体外诱导分化为神经元样细胞
- Author:
Hong XU
;
Xifeng MA
;
Lirong RUAN
;
Bo YANG
;
Yin DU
- Publication Type:Journal Article
- Keywords:
cord blood;
mesenchymal stem cells;
neuron-like cell;
induction differentiation
- From:
Basic & Clinical Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The mononuclear cells(MNCs) were cultivated and expanded into mesenchymal stem cells(MSCs) from human umbilical cord blood,and the purpose of this study was to explore the biological characteristics and induced differentiation ability in vitro.Methods Human umbilical cord blood samples were obtained and the mononuclear cells were isolated from it,then inoculated the MNCs into 25-mm culture flasks containing DMEM/F12 medium.The morphology was observed under microscope.Nissl body staining was used,The passage 2,4,7 of the expanded MSCs were induced to differentiate to neuron-like cells.The expressions of nestin and neuron-specific enolase (NSE) on the treated cells were detected by immunocytochemical method.Results Nissl body staining was positive;Nestin expression was found in(51.2?3.2)% of the second,(34.6?2.7)% of the fifth,(11.3?3.3)% of the seventh passage of MSCs;NSE expression was found in(11.4?2.3)% of the second,(21.78?3.1)% of the fifth,(40.7?3.4)% of the seventh passage of MSCs.Conclusion Cord bloodMSCs possess some features of neural stem cells,and have the capacity to differentiate into neuron-like cells under proper conditions.
