Effect of 5-Aza-CdR on RUNX3 expression and the proliferation of human hepatocarcinoma cell line HepG2
- VernacularTitle:5-氮杂-2’-脱氧胞苷对HepG2细胞RUNX3基因表达及细胞增殖的影响
- Author:
Xueyan ZHANG
;
Xu ZHANG
- Publication Type:Journal Article
- Keywords:
human hepatocarcinoma cell line HepG2;
RUNX3 gene;
demethylating agent
- From:
Basic & Clinical Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investingate the effect of 5-Aza2'-deoxycytidine(5-Aza-CdR)on cell growth and to explore the possibility of re-expression of the hypermethylated and silenced RUNX3 gene in hepatocarcinoma cell line HepG2.Methods The change in expression of the tumor suppressor gene RUNX3 mRNA in cultured HepG2 cells was observed by RT-PCR before and after 5-Aza-CdR treatment.Activity of cell growth was observed by MTT assay and colony-forming test.The cell cycle was analyzed by flow cytometry.Apoptotic morphology was observed by transmitting electron microscopy.Results The gene was reactivated by two different doses of 5-Aza-CdR treatment in HepG2 cell without expressing RUNX3.The hepatocarcinoma cell line treated with 5-Aza-CdR displayed a slowed growth rate in contrast to the control group.The colony formation rate of HepG2 cell treated with 5-Aza-CdR decreased dramatically(P
