Primary Analysis of Biochemical Characters of a Selenium-containing Protein From Se-enriched Ganoderma lucidum(Se-GL-P)
- VernacularTitle:富硒灵芝硒蛋白(Se-GL-P)生化性质的初步分析
- Author:
Ming DU
;
Xiaosong HU
;
Cong WANG
;
Guanghua ZHAO
- Publication Type:Journal Article
- Keywords:
Se-enriched Ganoderma lucidum, selenium-containing protein, Se-GL-P, character, analysis
- From:
Progress in Biochemistry and Biophysics
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
As a basic work of illustrating the relationship between the structure and function of a kind of protein purified from Se-enriched Ganoderma lucidum (Se-GL-P), the primary biochemical characters of Se-GL-P were characterized. Molecular mass was determined by two methods of sodium dodecyl sulfate polyacrylamide gel electrophoresis and size exclusion chromatogram. Amino acids composition, isoelectric point, peptide mass fingerprint, and the sequence of N terminal amino acids were determined by high performance liquid chromatography, capillary isoelectric focusing method, in-gel digestion, and Edman digestion method respectively. Results showed that, the Mr of Se-GL-P was 36 600, pI=4.01, and the sequence of N-terminal amino acids was DINGGGATLPQKLYLTPDVL. A conclusion was drawn that Se-GL-P was a kind of new selenium-containing protein, and it was one member of DING protein family.
