Expression of ERp29 in model of Parkinson's disease of PC12 cells induced by proteasome inhibitor
- VernacularTitle:PSI诱导PC12细胞帕金森病模型中ERp29蛋白表达
- Author:
Yu ZHANG
;
Hui XU
;
Ying ZHANG
;
Wei HAN
;
Yihong HU
;
Linsen HU
- Publication Type:Journal Article
- Keywords:
endoplasmic retuclum protein 29;
endoplasmin reticulum stress;
Parkinson's disease;
ubiquitin-proteasome system
- From:
Journal of Jilin University(Medicine Edition)
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the pathogenetic mechanism of ubiquitin-proteasome dysfunction in a model of Parkinson's disease(PD),which can provide the theoretical basis for PD.Methods After establishment of PD model induced by PSI in PC12 cells,proteins of untreated(DMSO) and PSI-treated PC12 cells were extracted 36 h after incubation,and then the maps of the extracted proteins were established by DIGE system.The altered protein spots were identified with MALDI-TOF Pro MS and database searching.Results Thirty-six treatment of PC12 cells with PSI induced the appearance of cytoplasmic Lewy body-like eosinophilic inclusions and apoptosis.The percentage of apoptotic cells was 25.53%.ERp29 were identified by MALDITOF Pro MS.The expression of ERp29 decreased in treatment group,compared with normal group(P
