Inhibitory effect of sodium phenylbutyrate combined with gefitinib on PKC of NB4 cells
- VernacularTitle:苯丁酸钠联合吉非替尼增强抑制NB4细胞的PKC信号传导途径
- Author:
Xu WANG
;
Wei LI
;
Guanjun WANG
- Publication Type:Journal Article
- Keywords:
sodium phenylbutyrate;
gefitinib;
drug therapy,combination;
NB4;
protein kinase C
- From:
Journal of Jilin University(Medicine Edition)
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of sodium phenylbutyrate(SPB) combined with gefitinib on NB4 cells.Methods The NB4 cells were divided into 8 groups randomly:control group,PMA group,H-7 group,SPB+PMA group,gefitinib group,gefitinib+PMA group,SPB+gefitinib group.The inhibitory rate of medicine was detected by MTT,the apoptosis of NB4 cells was detected by flow cytometry,the expressions of PKC,CDK,and P53 were detected with Western blotting method.Results The NB4 cells were suppressed obviously.The rate of growth inhibition and proliferation index in SPB+gefitinib group were 92.8% and 5.96%,those in SPB group were 45.1% and 29.6%,and gefitinib group were 61.0% and 35.9%,the differences were significant(P
