Reversal effect of Tan microemulsion on multidrug resistance
- VernacularTitle:微乳丹参酮逆转肿瘤多药耐药的作用
- Author:
Changjian LIU
;
Jianguo LIU
;
Tiecheng YU
;
Zongjian LUO
- Publication Type:Journal Article
- Keywords:
tanshinone;
microemulsion;
K562 cells;
genes,bcl-2;
p-glycoprotein;
drug resistance,multiple
- From:
Journal of Jilin University(Medicine Edition)
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the reveral effect of Tanshitone(Tan) Microemulsion on multidrug resistance(MDR) of sensitive K562 cell line(KS) and ADM resistant K562 cell line(KA) and the mechanism.Methods The reversal effect of Tan Microemulsion(Tan and Microemulsion as controls) on MDR of KA cells was observed.The inhibitory effect of cell growth was investigated by MTT.The apoptosis of KS and KA cell lines was observed by flow cytometry.The intracellular drug concentration was measured by detecting fluorescent density.Results The result showed that Tan Microemulsion,Microemulsion and Tan reduced the expression levels of P-gp and Bcl-2 in KA cells and Tan Microemulsion had the most significant effect.The MDR reversal folds of Tan Microemulsion of KA was highest among all 3 groups(P
