The effect of CD151 on Akt-Ser~(473) phosphorylation and human umbilical veine endothelial cell proliferation
- VernacularTitle:CD151对脐静脉内皮细胞Akt-Ser~(473)位点磷酸化和细胞增殖的影响
- Author:
Zhenzhong ZHENG
;
Zhenxiang LIU
- Publication Type:Journal Article
- Keywords:
CD151;
Ser/Thr protein kinase B;
Phosphorylation;
Human umbilical veine endothelial cell;
Cell proliferation
- From:
Journal of Medical Postgraduates
2003;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the the effect of CD151 on Akt-Ser473 and human umbilical veine endothelial cell proliferation.Methods:To construct pAAV-CD151 and transiently transfect to HUVEC(human umbilical veine endothelical) mediated with Lipofectamine 2000.HUVEC were assigned to control group,GFP group,CD151 group.After transfection,the expression of CD151,Akt and phospho-Akt were measured by western blot.Proliferation of HUVEC were measured by MTT colorimetric assay.Results:①The expression of CD151,phospho-Akt of pAAV-CD151 transfected group were significantly increased compared with non-transfected group and pAAV-GFP transfected group(P
