Effect of artemether on the poliferation of human lung adenocarcinoma cell line A549
- VernacularTitle:蒿甲醚对人肺腺癌A549细胞体外生长的影响
- Author:
Yan GUO
;
Jun WANG
;
Bicheng ZHANG
;
Zhengtang CHEN
- Publication Type:Journal Article
- Keywords:
Lung cancer;
Artemether;
Cell apoptosis
- From:
Journal of Medical Postgraduates
2003;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of antimalarial artemether on the proliferation of human lung adenocarcinoma cell line A549 in vitro and provide experimental data for the treatment of lung cancer with artemether.Methods:MTT assay was used to observe the inhibitory effects of artemether on the proliferation of A549 cells.Cell growth curve was draw according to the cell counts.The population doubling time was obtained in logarithmic growth phase.Cell cycle detection was observed by flow cytometry.H-E staining and transmission electron microscopy were used to observe the altered morphology of apoptotic cells. Results:Artemether has a significantly inhibitory effect on the proliferation of A549 cells in a dose-dependent manner in vitro,and the IC50 was 1.34 mg/L.The population doubling time in logarithmic growth phase in the artemether treatment group was(20.7?0.5) h compared to(32.2?0.3) h in the control group.The difference between two groups was statistically significant(P
