Experimental study of rat bone marrow stromal cell proliferation and differentiation in vitro
- VernacularTitle:大鼠骨髓基质细胞体外增殖分化的实验方法研究
- Author:
Fang ZHU
;
Guocheng ZHANG
;
Dongliang XU
- Publication Type:Journal Article
- Keywords:
Bone marrow stromal cells;
Proliferate;
Differentiate;
Neural growth factor
- From:
Journal of Medical Postgraduates
2003;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the conditions of in vitro proliferation and differentiation of rat bone marrow stromal cells(MSC) for its application in transplantation.Methods:MSC were obtained by flushing the shaft with DMEM using a sterile syringe,dissociated by density gradient centrifuge,and cultured in the medium containing DMEM and 10% fatal bovine serum(FBS).Then we added neural growth factors into the medium and observed their influence on MSC proliferation,differentiation and survival by phase contrast microscopy.Immunocytochemistry was used to identify cell types.Results:MSC cultured in medium containing DMEM/ 10% FBS/ neural growth factors proliferated quickly.The neuron-like cells appeared at 7-9 d with the expression of neurone specific enolase(NSE) at(52?9.2)% and glial fibrillary acidic protein(GFAP) at(15?7)%,and could survive more than 7 days;In contrast,cells induced by ?-mercaptoethanol(BME) died gradually after 24 hours.Conclusion:MSC can not only proliferate and differentiate into neuron-like cells effectively in the medium containing neural growth factors but also survive longer.
