Convenient preparation of mouse model of retinal neovascularization
- VernacularTitle:小鼠视网膜新生血管模型的简易制备
- Author:
Chunyan XUE
;
Zhenping HUANG
;
Jie JIN
- Publication Type:Journal Article
- Keywords:
Neovascularization,retina;
Mouse;
Vascular endothelial growth factor
- From:
Journal of Medical Postgraduates
2003;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the mouse model of retinal neovascularization. Methods:Twenty neonatal C57BL/6J mice were divided into two groups randomly.The treated group was exposed to 75% oxygen for 5 days and then to room air.All animals were sacrificed at postnatal day 17 and both eyes were enucleated.Endothelial cell nuclei extending into the internal limiting membrane were counted via HE stain and the expression of vascular endothelial growth factor(VEGF) was detected via immunohistochemical stain. Results:On average,25.70?4.90 endothelial cell nuclei were seen in the treated group and 0.85?1.02 in the control group.The difference was statistically significant(P
