Influence of katsutoxin and its extract on bone marrow colony-forming unit-granulocyte and monocyte following ~(60)Co gamma ray radiation
- VernacularTitle:蝎毒及其分离成分对~(60)Co ?射线照射后骨髓粒-单系祖细胞的影响
- Author:
Yongkui WANG
;
Ping DUAN
;
Guoling LI
;
Xuefei HAN
;
Weihua DONG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
The experiment was performed at Basic Medical College and First Affiliated Hospital, Zhengzhou University from September 2004 to December 2005. Totally 60 Kunming mice were divided into 5 groups randomly: ①blank control group (n =15) and simple radiation group (n =15). The mice were given 0.2 mL sterile saline by intraperitoneal injection. ②antineoplastic polypeptide from Buthus Martensii Venom (APBMV) group (n =10) and APBMV plus radiation group (n =10) received 0.2 mL APBMV according to prepared concentration by intraperitoneal injection. ③Katsutoxin extract Ⅲ plus radiation group (n =10) received 0.2 mL katsutoxin extract Ⅲ by intraperitoneal injection every other 5.5 hours for 7 days. After 24 hours from the last injection, the mice were endured 60Co g ray radiation (80 cm, 7.5 Gy irradiation dose, 0.27 Gy/min dose rate). Then katsutoxin extract Ⅲ was given same as above for 7 days. Then bone marrow was extracted to be cultured to colony-forming unit-granulocyte and monocyte (CFU-GM). The findings showed that colony amount of APBMV plus radiation group and katsutoxin extract Ⅲ plus radiation group was obviously more than that of simple radiation group [(32?5),(27?3),(2?1)pieces/well,P
