Synergism of Bactericidal Activities of Imipenem Combined with Azithromyicn in Treatment of Infection Associated with Pseudomonas aeruginosa Biofilm
- VernacularTitle:亚胺培南联合阿奇霉素治疗铜绿假单胞菌生物被膜感染的实验研究
- Author:
Xiangqun FANG
;
Youning LIU
- Publication Type:Journal Article
- Keywords:
Biofilm;
Glycocalyx;
Macrolides;
Imipenem
- From:
Chinese Journal of Nosocomiology
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the synergism of imipenem(IMP) combined with azithromyicn(AZM) in the treatment of infection associated with Pseudomonas aeruginosa biofilm.METHODS The inhibitory activity of AZM to glycocalyx(GLX) production was detected with the L-tryptophan method.Viable counts in biofilms treated with AZM combined with IMP were detected with the methyl thiazolyl diphenyltetrazolium(MTT) method in vitro.The tissue cage method was used to establish the animal model of local P.aeruginosa biofilm infection and the synergism of IMP combined with AZM was also studied in vivo.RESULTS AZM could inhibit the production of GLX.When IMP was combined with 1/4MIC or 1/16MIC of AZM viable counts in biofilms were less than they were when IMP was given alone in vitro.When AZM and IMP were administered at the same time viable counts in tissue cage fluids were also less than they were when IMP was administered alone in vivo.CONCLUSIONS AZM could enhance the antibacterial activity of IMP in the treatment of infection associated with biofilm.
