ESBLs Produced by Enterobacter cloacae
- VernacularTitle:阴沟肠杆菌流行菌株产超广谱?-内酰胺酶分析
- Author:
Deping DONG
;
Lizhong HAN
;
Shilin CHEN
;
Yuxing NI
- Publication Type:Journal Article
- Keywords:
Enterobacter cloacae;
ESBLs;
CTX-M
- From:
Chinese Journal of Nosocomiology
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the ESBLs produced by clinical strains of Enterobacter cloacae.METHODS Production of ESBLs was identified with modification of the double-disk test(MDDT).Eight kinds of primers(CTXM,CTXM-1,CTX-M-2,CTX-M-9,SHV,TEM,VEB,and PER)were used for the PCR amplification.Gene clone and DNA sequencing were performed then.RESULTS The result of MDDT was positive,amplicons were gained by PCR amplification with CTXM-1.DNA sequencing of amplicons of this strain revealed ESBLs of CTX-M-3.CONCLUSIONS ESBLs are the important reason for E.cloacae resisting to the third-generation cephalosporins.
