DNA Typing of Staphylococcus epidermidis with Randomly Amplified Polymorphic DNA
- VernacularTitle:RAPD技术对表皮葡萄球菌DNA分型
- Author:
Meirong ZHOU
;
Dafang WANG
;
Rirong CHEN
;
Yuxing NI
- Publication Type:Journal Article
- Keywords:
Staphylococcus epidermidis;
RAPD
- From:
Chinese Journal of Nosocomiology
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To type Staphylococcus epidermidis and analyze its polymorphism by randomly amplified polymorphic DNA(RAPD),and provide the method for analysis of homogeneity of S.epidermidis.METHODS Twenty one collected strains from ophthalmology ward were analyzed using random primer RAPD1 with RAPD.RESULTS On the basis of bands,difference of clinical strains was analyzed.Twenty one strians of S.epidermidis were divided into 5 patterns.Among them type Ⅰ taken 71 percent(15/21).CONCLUSIONS Typing of S.epidermidis by RAPD at molecular level is very rapid,easy and reliable.It can apply to track pathogens of the hospital and study epidemiology of the bacteria.
