Screening and identification of differentially expressed genes in colorectal carcinoma
- VernacularTitle:结肠癌组织中差异表达基因的筛选和鉴定
- Author:
Chunling HUANG
;
Zhongying ZHANG
;
Zhjie DING
;
Qi LUO
- Publication Type:Journal Article
- Keywords:
Colorectal carcinoma;
mRNA differential display;
gene expression
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen and identify differentially expressed genes in colorectal carcinoma and explore the possible molecular pathogenesis of colorectal carcinoma.Methods The differentially expressed cDNA bands in colorectal carcinoma specimens and matched adjacent normal tissues were isolated by fluorescent mRNA differential display.Following differential display PCR (DD-PCR),all cDNA fragments were sequenced.By using BLAST software,the sequencing results were compared with Genebank database for homologue analysis.RT-PCR was used to detect the expression of one of the differentially expressed genes in colorectal carcinoma samples were identified by semi-RT-PCR.Results BLAST analysis revealed that the cDNA band was homologous to DDX32 (99%) and its up-regulated expression in colorectal carcinoma tissues was confirmed by RT-PCR (P
