Detection of rpsL drug-resistant gene in L-form of Mycobacterium tuberculosis by PCR-SSCP
- VernacularTitle:PCR-SSCP法检测结核分枝杆菌L型rpsL耐药基因
- Author:
Jun LU
;
Song YE
;
Qiang JIN
- Publication Type:Journal Article
- Keywords:
pneumoconiosis;
Mycobacterium Tuberculosis L-form;
drug-resistance;
rpsL;
polymerase chain reaction and single-strand conformation polymorphism (PCR-SSCP)
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between mutation of rpsL drug-resistant gene in L-forms of Mycobacterium tuberculosis and drug-resistance to streptomycin in pneumoconiosis patients.Methods A total of 52 clinical isolated strains of Mycobacterium tuberculosis L-forms were collected from 97 pneumoconiosis patients.The mutation of rpsL gene was detected by PCR-SSCP,and the drug-resistance to streptomycin was performed by routine antimicrobial susceptibility test(AST).Results The results of drug susceptibility test showed that 26 in the 52 clinical isolated strains were drug-resistant to streptomycin.The streptomycin-resistant rate was 50.00%(26/52).The gene mutation rate of rpsL detected by PCR-SSCP was 40.38%(21/52).The coincidence rate of two experimental results was 80.77%(21/26).Conclusion High detectable rate of streptomycin-resistant strains in Mycobacterium tuberculosis L-forms was found by PCR-SSCP.The application of PCR-SSCP may possess important value for guiding clinical medication of pneumoconiosis patients complicated with tuberculosis among coal workers
