Preliminary study on blood proteome processing and mass spectrometry standards
- VernacularTitle:血液蛋白质组与质谱仪检测流程标准化初探
- Author:
Jiandong LIU
;
Yongzhe LI
;
Ning LI
;
Weixing LI
;
Yang XU
- Publication Type:Journal Article
- Keywords:
magnetic beads;
protein fingerprinting technology;
mass spectrometry
- From:
Basic & Clinical Medicine
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of sampling,handling,shipping and storage on low-molecular-weight serum proteome profiling.Methods Serum samples instantly separated and aliquoted,some stored at-80 ℃ for up to 6 months,others stored at 4 ℃ or room temperature(25 ℃) for 2 to 72 hrs.The variations of protein profiling under these conditions on WCX magnetic beads were studied.Profiling influenced by hemolysis,multi freeze-thaw cycles,storage conditions.Results Different handling procedures and storage conditions have different effects on serum profiling.Serum stored at-80 ℃ up to 6 months,stored at 4 ℃ or 25 ℃ for 2 h or one freeze-thaw cycle,had little effects on serum proteomic analysis.If serum diluted into 9 mol/L urea buffer at room temperature,the result is stable for 24 hours.Statistics analysis shows that 35.9% peaks have significant changes(P
