cDNA Cloning and Sequence Analysis of Human Bactericidal/Permeability-increasing Protein
- VernacularTitle:人细菌透性增加蛋白cDNA的克隆及序列分析
- Author:
Hairong MA
- Publication Type:Journal Article
- Keywords:
Human bactericidal/permeability-increasing protein;
cDNA;
TD-PCR;
Gene cloning;
DNA sequence analysis
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Human bactericidal/permeability-increasing protein(hBPI)cDNA was amplified by reverse transcription(RT)and touchdown PCR(TD-PCR)from blood stem cells collected from healthy human of Uygur nationality in Xinjiang Uygur Autonomous Region of China, and then was subcloned into pEGFP-N1 vector,hBPI cDNA sequence consists of 1,464bp.Comparison with other 4 hBPI cDNA sequences registered in GenBank identified 99% homology in DNA sequence.However,there were two base substitutions(nucleotide 576G→C,nu- clotide 676A→G),one of which resulted in an amino acid(residue 185 Lys→Glu).
