Isolation,Identification and Molecular Characterization of a Velogenic NDV Isolate from a Broiler Flock in High Antibody Level Against NDV
- VernacularTitle:高抗体水平种鸡NDV的分离鉴定和分子特性
- Author:
Baochen MA
- Publication Type:Journal Article
- Keywords:
Newcastle disease virus;
High antibody level;
Molecular characterization
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
A Newcastle disease virus(NDV)field strain SGM01 was isolated from a broiler flock in high antibody level against NDV,and identified by HA,HI cross test and animal regression.SGM01 was determined as a virulent strain with MDT of 50.5h,ICPI of 1.76,IVPI of 2.41 after plaque-purification.The F and HN gene of SGM01 were cloned and sequenced.Analysis of F gene indicated that SGMOI be- longed to genotypeⅦ.The amino acid sequence ~(111)GGRQGRL~(117) in the F protein cleavage site in SGM01 strain is identical to virulent NDV. The homology analysis of F and HN gene sequences compared to reference strains from GenBank indicated that:The F protein amino acid se- quence has homologies of 87.7%～88.3% with published gene typeⅡstrains LaSota and SQZ04 et al.,95.7%～98.2% with genotypeⅦstrains Taiwan95,Yunnan03 et al.,91.8%～91.7% with genotypeⅨstrains F_(48)E_9 et al..Willie HN genes were compared,SGM01 demonstrated a higher homologies of 96.5%～97.2% with strains which were isolated in recent years,but lower homologies of 87.4% and 89.0% with LaSota and F_(48)E_9.
