Studies on Mechanism of Protective Immunity Against Infection of Schistosoma japonicum Induced by Sj26 Gene Transfected Dendritic Cell
- VernacularTitle:Sj26基因转染的树突状细胞对日本血吸虫感染的免疫保护机制研究
- Author:
Dingwen SHEN
;
Jinping LUO
;
Yonglong LI
;
Wenqi LIU
;
Xiaochun LONG
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Dendritic cell;
Sj26 gene;
Gene transfection;
Protective immunity
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of protective immunity against Schistosoma japonicum infection induced by Sj26 gene transfected dendritic cell(DC).Methods 48 BALB/c mice were divided randomly into 4 groups with 12 each.The mice were injected through auricle for three times with Sj26 gene transfected DC(Group A),pcDNA3 transfected DC(Group B),untreated DC(Group C) and RPMI-1640(Group D) respectively,and challenged with 40?2 cercariae of S.japonicum per mouse 2 weeks after the last immunization.Sera from mice were examined for IgG antibody,IFN-? and IL-4 by ELISA.Western blot was used for detecting specific anti-Sj26 IgG antibody.The production of IFN-? and IL-4 in the supernatant of spleen cells stimulated with soluble egg antigen(SEA) and ConA was quantified by sandwich ABC-ELISA.The proliferation of spleen cells were measured with MTT method.Results IgG antibody increased significantly in the mice of group A at 2 weeks after the last immunization(absorbency A491=0.117),higher than that of group B(A491=0.061) and group C(A491=0.058)(P
